Salmonella Latex Agglutination Kit

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Microgen Salmonella Latex

A latex agglutination confirmation test for the presence of Salmonella spp on solid media from clinical and food samples. Accurate enough to screen positives from 24 hour enrichment broths. Detects motile and non motile strains. Reduces testing delays associated with conventional methods. The Salmonella latex offers a rapid alternative to the traditional identification methods and detects a large range of Salmonella species including. S.typhimurium, S.enteritidis, S.virchow, S.newport, S.infantis, S.anatum, and various others belonging to serogroups B, C1, C2, C3, D1 and E1. Benefits
Results in 2 minutes
Cost effective
High sensitivity
High specificity
Accurate & reliable results
User friendly procedure

Included in the kit:

•  2.5mls Test latex
•  0.5ml Positive control
•  5mls Isotonic Saline
•  disposable reaction cards
• disposable mixing sticks

   

Method from plates

1.  place 1 drop of saline on to a well of the reaction card
2.  remove a typical looking colony from the plate using a loop and emulsify in the drop of saline
3.  rock the slide gently for 2mins to check for autoagglutination
4.  add 1 drop of test latex to the suspension, mix using a mixing stick
5.  rock the slide for up to 2 minutes and examine for agglutination

Interpretation

•  agglutination within 2 minutes is indicative of the presence of Salmonella spp in the sample
•  absence of agglutination indicates that Salmonella spp are not present in the sample

Background

The genus Salmonella consists of >2000 serotypes. Can cause acute infectious enteritis frequently associated with food poisoning. Many Salmonella species infect domestic animals clinically or sub-clinically therefore many cases of Salmonella food poisoning originate from animal sources. In contrast S.typhi and S.paratyphi only infect humans and cause enteric fever. Salmonellae are usually present in samples in low numbers therefore enrichment or selective media are used to promote their growth eg RV, SC, M- broth

 Latex Agglutination Principle

•  Polyvalent antisera raised against a variety of Salmonella flagella antigens is used to coat latex particles.
•  When mixed with a suspension of Salmonella containing these antigens, the latex particles rapidly agglutinate forming visible clumps
• Microgen Salmonella Latex test detects the majority of common species including S. typhimurium and S. enteritidis
• In addition, the non-motile S. gallinarum and S. pullorum  are also detected as they retain the protein basal bodies which are the attachment points for flagella

Latex technology:

•  Producer strains carrying specific antigens are cultured
•  Jameson plates used to purify strain to single phase as strains can be present in 2 flagella phases
•  strains cultivated to produce immunogen
•  immunogen used to generate antisera
•  antisera titres checked and blended
•  blended antisera purified and then coated on latex particles

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 Antisera Included

 Brief Evaluation 

 Comparison with Bactigen and Spectate

 Comparison with MUCAP test and Rambach agar 

 Effectiveness of Presumed ID

 Evaluation of Latex Agglutination Test 

 Evaluation of test with liquid and solid media 

 Improved ProtoCOL

 Salmonella Latex Brochure 

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