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Pathogen Identification Kits
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{tab=Features}
Gram Negative (GN) ID
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Microgen™ Gram Negative (GN) ID
Identifies the complete range of Enterobacteriaceae and other non-fastidious, oxidase positive and negative, gram negative bacilli. The GN-A kit includes 60 microwell strips in foil pouches, frame for microwell strips, result forms, instructions for use and color chart for reading results. GN-A kit can be used separately from GN-B kit. GN-B kit includes 24 microwell strips in foil pouches and instructions for use. GN-B kit id designed to be used together with GN-A kit and not on its own. Identification software and reagents must be purchased separately.
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Features list
- Simple to inoculate, handle and read
- Strip includes all tests in ISO and other standard methods
- No toxic reagents
- Results within 24 hours
- 100% sensitivity
- No MacFarland calibration
- Ability to isolate directly from chromogenic media
- No need for additional tests
- No assembly required
- No weak color changes
- Cost efficient
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Easy to use:
- Select a single colony of the isolate to be identified
- Emulsify colony in 3ml saline (A) or 5ml saline (A+B)
- Add 3-4 drops (100µL) of the suspension to each well of the strip(s)
- Overlay appropriate wells with mineral oil
- Incubate 18-24 hours at 35-37°C
- Next day add reagents
- Read and Record results using the color chart (provided) and Identification Software (purchased separately)
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| The GN-ID system comprises two separate, 12-substrate, microwell strips; the GN-A microwell strip and the GN-B microwell strip. The GN-A strip can be used separately and identifies the most commonly encountered, oxidase negative, non-fastidious, gram negative bacilli. GN-B is designed to be used in conjunction with GN-A; the combined strips identify the complete range of Enterobacteriaceae and other non-fastidious, oxidase positive and negative, gram negative bacilli. The wells in each microwell strip contain dehydrated biochemical substrates that are reconstituted with a saline suspension of the organism to be identified. If the substrate found in each well is metabolized by the organism, then a color change occurs during incubation or after the addition of specific reagents. Substrates are based on conventional biochemical methods used for identifying Enterobacteriaceae and other gram negative bacilli. |
| Procedure
A pure, 18-24 hour culture of the isolate to be identified must always be used. We suggest selecting a single colony from BD CHROMagar Salmonella, BD Trypticase soy agar, BD Bismuth Sulfite Agar or other plated media. An oxidase test must be performed on the isolate prior to strip inoculation. Oxidase positive organisms can only be identified when A and B strips are both inoculated. Emulsify a single colony from an 18-24 hour culture in sterile physiological saline (3 ml for strip A, 3-5 mls for A+B). Mix thoroughly. Add 3-4 drops (100µL) of the suspension to each well of the strip(s). After inoculation, overlay specified wells with 3-4 drops of mineral oil; these wells are highlighted with a black circle around the well to assist in adding oil to the correct wells. Seal the top of the microwell strip(s) with adhesive strip provided and incubate at 35-37oC for 18-24 hours. Remove adhesive strip and record all positive reaction with the aid of the color chart. Add Indole Kovacs, VP I & II, Nitrate A & B and TDA reagents to the appropriate wells as described in the Instructions for Use. Read and record results using the color chart and Identification Software (sold separately). For a complete explanation of procedure for use, please refer to the Instructions for Use, included with your kit.
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Step 1: Select a single colongy
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Step 2: Emulsify in saline solution
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Step 3: Inoculat strip with solution
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Step 4: Add mineral oil
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Step 5: Apply adhesive strip & incubate |
Step 6: Next day, add reagents |
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Step 7: Read and record results
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{tab=Literature}
 Color Chart GNA
GN ID Brochure
GN ID A&B Users Guide
Guide carbohydrate fermentation
Guide citrate utilization reaction
Guide Decarboxylase
Guide gelatin liquefaction
Guide hydrogen sulphide production
Guide Indole production
Guide malonate inhibition
Guide ONPG+Nitrate reduction tests
Guide taxonomic changes
Guide tryptophandeaminase
Guide Urease production
Guide Voges proskauer reaction
Microgen vs.microbact enterobacteriaceae
Performance of GNA with CHROMagar Salmonella
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