BBL CHROMagar Staph aureus

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Chromogenic Media

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staphBBL™ CHROMagar™ Staph

BBL™ CHROMagar™ Staph aureus medium is designed for the isolation and identification of Staphylococcus aureus without the use of confirmatory testing from clinical sources. BBL CHROMagar Staph aureus medium can be utilized for the isolation and identification of S. aureus from food and environmental sources. BBL CHROMagar Staph aureus is highly effective in differentiating organisms with atypical appearance or weak hemolysis making it an ideal medium for Staphylococcus aureus surveillance.

Enjoy the following features and benefits with BBL™ CHROMagar™ Staph:

  • AOAC- RI approved
  • Used as primary plate
  • Time to results 24 hours
  • Confirmatory detection of Staphylococcus aureus
  • Increase recovery by 11% compared to Mannitol Salt agar,
  • perform susceptibility testing directly
  • Sensitivity 99.5%
  • Specificity 99.2%

Results

After proper incubation, read plates against a white background. S. aureus produces mauve to orange/mauve colored colonies and Staph saprophyticus appear green to blue-green on the CHROMagar medium. Most gram-positive organisms, if not inhibited, will produce white, blue, green or blue-green (aqua) colonies. Gram-negative organisms and yeasts are partially to completely suppressed.

Principles

Specially selected peptones supply the nutrients in BBL CHROMagar Staph aureus medium. The chromogen mix consists of artificial substrates (chromogens), which release an insoluble colored compound when hydrolyzed by specific enzymes. Selective agents have also been added for the suppression of gram-negative organisms and partial suppression of yeast.

Procedure

1. Enrich sample in enrichment broth i.e. peptone buffered broth at 35 degrees C. for 24 hours.

2. Inoculate CHROMagarTM Staph plate with 0.1ml of the enrichment broth and streak for isolation. Incubate at 35 degrees C. for 24 hours. If the specimen is cultured from a swab, roll the swab over a small area and streak from this area with a loop.

3. Observe plates for characteristic colonial morphology and color at 18-24 hours. If negative for Staph re-incubate for an additional 12-24 hours and read again.

AOAC is a trademark and Performance Tested Methods Program is a service mark of AOAC International. CHROMagar is a trademark of Dr. A. Rambach. BD, BD Logo and BBL are trademarks of Becton, Dickinson and Company. ©2005 BD

 

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pdfGeneral Brochure BD CHROMagar

pdfCHROMagar Staph Brochure

pdfStaph Flowchart

 

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BBL CHROMagar Staph aureus
Storage:
 Upon receipt store at 2-8 degrees C. away from direct light. Product is light and temperature sensitive; protect from light, excessive heat and freezing. The expiration date applies to the product in its intact packaging when stored as directed. This product has a 90 day shelf life from the date of manufacture.

 

 

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