BBL CHROMagar Salmonella

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BBL™ CHROMagar™ Salmonella

BBL CHROMagar Salmonella is a selective and differential medium for the isolation and presumptive identification of Salmonella species from a variety of food products. BBL CHROMagar Salmonella has been validated by the AOAC Research Institute (AOAC™-RI) under the Performance Tested MethodsSM Program

CHROMagar Salmonella^TM provides:

• AOAC-RI approved
• 100% correlation to official methods (USDA, FDA and ISO)
• Presumptive identification of Salmonella species
• 50% reduction in plated media costs versus official methods
• Time to detection as early as 24 hours from enrichment broth
• Final read for negativity at 48 hours

Results

After proper incubation, read plates against a white background with good lighting. Salmonella enteritidis and other Salmonella species will appear as light mauve to mauve-colored (rose to purple) colonies. Citrobacter freundii an other coliforms will appear as light blue-green to blue-green colored colonies. Some organisms that do not hydrolyze any of the chromogenic compounds may appear as colorless colonies.

Principles

BBL CHROMagar Salmonella is intended for the isolation and differentiation of Salmonella species based on the formation of an insoluble pigment. The addition of chromogenic substrates in the medium facilitates detection of Salmonella species from other normal flora. Unlike traditional media, BBL CHROMagar Salmonella does not detect Salmonella based upon H₂S or glucuronidase production, or on non-lactose fermentation. This unique BBL formulation allows Salmonella species to produce mauve (rose to purple) colonies that are easily differentiated from other bacteria, including coliforms, which may resemble Salmonella on other traditional media.

Procedure

1. Enrich sample in enrichment broth i.e. buffered peptone water, Salmonella preenrichment broth, at 35 degrees C. for 24 hours.

2. Inoculate CHROMagar^TM Salmonella plate with 0.1ml of the enrichment broth and streak for isolation. Incubate at 35 degrees C. for 24 hours. If the specimen is cultured from a swab, roll the swab over a small area and streak from this area with a loop.

3. Observe plates for characteristic colonial morphology and color at 18-24 hours. If negative forSalmonella, re-incubate for an additional 12-24 hours and read again.

AOAC is a trademark and Performance Tested Methods Program is a service mark of AOAC International. CHROMagar is a trademark of Dr. A. Rambach. BD, BD Logo and BBL are trademarks of Becton, Dickinson and Company. ©2005 BD

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General Brochure BD CHROMagar

BD Catapult Summer 2005

Salmonella Flowchart

CHROMagar Salmonella Brochure

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